Influences of Xeno-Free Media on Mesenchymal Stem Cell Expansion for Clinical Application

Abstract Mesenchymal stem/stromal cells (MSCs) are multipotent somatic stem/progenitor cells that can be isolated from various tissues and have attracted increasing attention from the scientific community. This is due to MSCs showing great potential for incurable disease treatment, and most applications of MSCs involve tissue degeneration and treatment of immune- and inflammation-mediated diseases. Conventional MSC cultures contain fetal bovine serum (FBS), which is a common supplement for cell development but is also a risk factor for exposure to animal-derived pathogens. To avoid the risks resulting from the xenogeneic origin and animal-derived pathogens of FBS, xeno-free media have been developed and commercialized to satisfy MSC expansion demands for human clinical applications. This review summarized and provided an overview of xeno-free media that are currently used for MSC expansion. Additionally, we discussed the influences of different xeno-free media on MSC biology with particular regard to cell morphology, surface marker expression, proliferation, differentiation and immunomodulation. The xeno-free media can be serum-free and xeno-free media or media supplemented with some human-originating substances, such as human serum, human platelet lysates, human umbilical cord serum/plasma, or human plasma-derived supplements for cell culture medium. These media have capacity to maintain a spindle-shaped morphology, the expression of typical surface markers, and the capacity of multipotent differentiation and immunomodulation of MSCs. Xeno-free media showed potential for safe use for human clinical treatment. However, the influences of these xeno-free media on MSCs are various and any xeno-free medium should be examined prior to being used for MSC cultures.

Influences of Xeno-Free Media on Mesenchymal Stem Cell Expansion for Clinical Application

Abstract Mesenchymal stem/stromal cells (MSCs) are multipotent somatic stem/progenitor cells that can be isolated from various tissues and have attracted increasing attention from the scientific community. This is due to MSCs showing great potential for incurable disease treatment, and most applications of MSCs involve tissue degeneration and treatment of immune- and inflammation-mediated diseases. Conventional MSC cultures contain fetal bovine serum (FBS), which is a common supplement for cell development but is also a risk factor for exposure to animal-derived pathogens. To avoid the risks resulting from the xenogeneic origin and animal-derived pathogens of FBS, xeno-free media have been developed and commercialized to satisfy MSC expansion demands for human clinical applications. This review summarized and provided an overview of xeno-free media that are currently used for MSC expansion. Additionally, we discussed the influences of different xeno-free media on MSC biology with particular regard to cell morphology, surface marker expression, proliferation, differentiation and immunomodulation. The xeno-free media can be serum-free and xeno-free media or media supplemented with some human-originating substances, such as human serum, human platelet lysates, human umbilical cord serum/plasma, or human plasma-derived supplements for cell culture medium. These media have capacity to maintain a spindle-shaped morphology, the expression of typical surface markers, and the capacity of multipotent differentiation and immunomodulation of MSCs. Xeno-free media showed potential for safe use for human clinical treatment. However, the influences of these xeno-free media on MSCs are various and any xeno-free medium should be examined prior to being used for MSC cultures.

Blunt liver injuries in children: the role of nonoperative management

Background: Nonoperative management (NOM) is presently considered the treatment modality of choice for hemodynamically stable patients sustaining blunt liver trauma, especially in children. Objective: To evaluate role of NOM of blunt liver injuries (BLI) in children at National Hospital of Pediatrics. Subjects and method: Therecords ofallthe patientstreatedin National HospitalofPediatricswithfinaldiagnosisBLI betweenJanuary2000 and December 2006 were reviewed. The clinical signs,investigations,imagingstudies, methods oftreatmentandresultswereanalysed.Theliverinjuriesweregradedaccordingtothe American AsociationfortheSurgeryofTrauma(AAST).Results: There were15patients from one dayto 12 years of age with average BLI grade 2.5; 14 patients with precise diagnosis BLI were atempted NOM, 1 neonate was operated with diagnosis intraabdominal haemorrhage (BLI grade II found intraoperatively). Two patients (BLI grade II and V) from the atempted NOM group were operated thereafter because of hemodynamical instability or continuing bleeding, the remained 12recovered well with average hospital stay of 7.5 days. The rate of succesful NOM for BLI in our series was 12/14 (85.7%). Conclusions: NOM can be applied safely for BLI in children with high successful rate. Patients\ufffd?hemodynamic status may be more important for treatment decision-making than the injury grade according to the AAST based on ultrasound or CT.

Results for cardiac intervention in National Hospital of Pediatrics

Background:The progress of the cardiac intervention technique has been changed the doctors' viewpoint in the treatment for congenital cardiac. In the past years, treatment for congenital cardiac needed to surgery but nowadays, the cardiac intervention technique are being applied in many Cardiac Centers with very good results. This technique also helped to prevent the complication in cardiac operation. Objectives:This study aims to report the results for cardiac intervention in National Hospital of Pediatrics. Subjects and method:A prospective study was conducted on 298 children diagnosed with patent ductus arteriosus, atrial septal defect (ASD), ventricular septal defect, coarctation, pulmonary stenosis (PS), aortic stenosis (AS), small patent ductus arteriosus (PDA) infant with pulmonary atresia - ventricular septal defect (VSD) and Dextro- transposition of the great arteries (d- TGA) with intact ventricular septum at National hospital of Pediatric between June 2004and October 2006. Results: Transcatheter closure of PDA, ASD by Amplatzer or Coil was safe and effective. Critically ill children diagnosed with PS, AS and Coarctation may be saved by valvuloplasty and angioplasty procedures. Catheter interventions avoided the needs for surgery without scars. Catheter interventions had low complications. Conclusion: The hospitalized duration was short so the expenditures reduced.

Efficacy of nonoperative management of blunt splenic injury in children

Background: Nonoperative treatment of blunt splenic injury in children has become routine standardization in Viet Nam. Objectives:This study aims to study the efficacy of nonoperative management (NOM) for blunt splenic injury (BSI) in children. Subjects and method: The records of all the patients treated in National Hospital of Pediatrics with final diagnosis blunt splenic injury between January 2000 and December 2006 were reviewed. The clinical signs, investigations, imaging studies, methods of treatment and results were analyzed. The splenic injuries were graded according to the American Association for the Surgery of Trauma (AAST). Results:There were 15 patients from one day to 12 years of age, with average injury grade 2.2. From 13 patients who were attempted NOM, only 2 patients (blunt liver injury - BLI grade 3 and 4) were operated thereafter because of continuing bleeding or worsening clinically, the remains of 12 recovered well with average hospitalized duration wasf 6 days. The NOM for BLI was successful in 11/13 (84.6%) in our series. Conclusion:NOM was highly efficient method and should be the standard initial approach for all the children with BLI. These patients must be closely monitored for prompt surgical treatment in case of NOM failure.

Results of the treatment of posterior urethral valve by endoscopic resection

The aim of the study is to investigate clinical and laboratory findings of posterior urethral valve, and to evaluate the result of management by endoscopic resection. During 3 years (1998-2000), 28 children were treated in National Institute of Pediatrics. The main clinical findings were dysuria and urinary pseudo incontinence. Voiding cystography was a tool for confirming the diagnosis. The endoscopic resection was a method of choice in the treatment.

Hyperinsulinemic hypoglycemia

This was the first time in Vietnam, an infant with Hyperinsulinemic glycemia has been surgical treated in the Institute of Mother and infant protection and care. Newborn with ages of 10 days admitted to hospital with diagnosis of Hyperinsulinemic glycemia. The results have shown that liver and spleen was normal, size and density of pancreas were normal without tumor, exocrine glands were normal, there was hyperplasia of island of Langerhans which generated the abnormal size, multinuclear cells or megakaryocytic with thick wall. 95% of pancreas was removed

Hirschsprung disease or congenital nonglandular digestive abnormality

Hirschsprung disease or congenital nonglandular digestive abnormality

Preliminary results of the treatment of Hirschprung's disease by survey through posterior sagittal approach

The aim of the study is to describe the technique of operation for Hirschprung's disease by a Soave modified procedure through the posterior sagittal approach, to propose the indications and to evaluate the early result. From January 2000 to April 2000, 26 children suffering from Hirschprung's disease were operated by this technique. There was no mortality during and after operation. The anastomosis leakage occurred in one patient. The posterior sagittal approach is a convenient and safe one in survey for Hirschprung's disease with the ganglionic segment involves in the rectum and sigmoid.

Livers transplantation for children suffering from congenital biliary atresia in the National Institute of Pediatrics

During 5 years (1994-1998), 147 children suffering from congenital biliary atresia were admitted to NIP. Clinical manifestations include icterus, discolored stool and hepatomegaly. The gallbladder is absent; ultrasonography shows that its diameter doesn't change before, during and after meal in all patients. 136 patients among those children require a liver's transplantation. It means that, every years, there were 27 children suffering from biliary atresia who require a liver's transplantation.

Undetermined gender (ambiguous genitalia)

Undetermined gender divided into 4 groups including female pseudohermaphroditism, male pseudohermaphroditism, true pseudohermaphroditism and others. This paper introduced the studies on the causes, diagnosis and treatment of 4 above mentioned groups of diseases. The approach methods of causative diagnosis of undetermined gender (ambiguous genitalia) including clinical approaches, chromosome tests, biochemical tests, genital tract X-ray and genital biopsy.

Meckel's diverticulum bleeding

During 6 years, 5 children suffering from gastrointestinal bleeding due to Meckel's diverticulum were diagnosed and operated in the National Institute of Pediatrics. Clinical manifestations include melena and abdominal pain. Scintigraphy is a reliable tool to confirm the diagnosis

Clinical and paraclinical features and the treatment of ureterocele

Between April 1992 and March 2001, a total of 52 patients (58 ureteroceles) was diagnosed and treated at National Pediatric Institute. For ureterocele, female to male ratio was 5.2:1. Complete duplication was found in association with the ureterocele in 50 cases. Urinary tract infection was the most frequent mode of presentation (64%), abdominal mass (12%) and prolapsed ureterocele (13%). The diagnostic procedure included ultrasonographic examination, voiding cystourethrography and excretory urogram. Of 52 patients, 82% and 77% were less than 2 years old at presentation and operation respectively. The elective surgical policy was individualized, based on renal function and presence of vesicoureteral reflux. Overall results within various groups were generally satisfactory regarding the eradication of urinary tract infection, the preservation of renal function in 73% cases after the first procedures